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| 발행년 : | 2015 |
|---|---|
| 구분 : | 국내학술지 |
| 학술지명 : | 韓國受精卵移植學會誌 |
| 관련링크 : | http://www.riss.kr/link?id=A101658319 |
- 저자명
김현,최창용,성환후
- 학술지명
韓國受精卵移植學會誌
- 권호사항
Vol.30 No.3 [2015]
- 발행처
한국수정란이식학회
- 발행처 URL
- 자료유형
학술저널
- 수록면
207-211(5쪽)
- 언어
Korean
- 발행년도
2015년
- 등재정보
KCI등재
- 초록 (Abstract)
- 목차 (Table of Contents)
- 서 론
- 재료 및 방법
- 1. 체외수정
- 2. 도입유전자의 구조와 조정
- 3. 수정란의 동결 및 융해
- 4. 마우스 전핵기란에 도입유전자의 미세주입과 배이식
- 5. 서던블럿 분석
- 6. Tg 마우스의 발현해석
- 7. 통계분석
- 결 과
- 고 찰
- 요 약
- REFERENCES
Many pronuclear stage eggs were used to generate transgenic mice (Tg) by microinjection. In this study, we used in vitro fertilized mouse eggs, followed by ultrarapid freezing to establish a simple procedure for production of Tg mice. We produced in vitro fertilized mouse eggs and cryopreserved them by ultrarapid freezing method. A total of 139 cryopreserved-thawed pronuclear eggs, of which 101 (72.6%) were survived following microinjection of chicken b-actin promoter-driven firefly improved luciferase cDNA (β-act/luc+) and were transferred into 5 recipients. All recipients became pregnant and gave birth to a total of 15 (14.8%) pups. As a control, same DNA construction (β- act/luc+) was also injected into 450 in vitro fertilized eggs, of which 338 (75.1%) were survived and then were transferred into 14 recipients. Eleven (78%) mice became pregnant and littered a total of 54 (19.1%) pups. Southern blotting analysis of Tg mice indicated that one (1/15, 6.6%) and three (3/54, 5.5%) transgenic mice were production from cryopreserved and in vitro fertilized eggs, respectively. All Tg mice produced from both eggs showed the expression of improved luciferase gene. These results indicated that efficiency of produced of Tg mice from cryopreserved eggs was comparable to that from in vitro fertilized eggs. Furthermore, it is suggested that microinjection of transgene into in vitro fertilized eggs cryopreserved by ultrarapid freezing is an easy and conveniently method for production of Tg mice.
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