Human embryonic stem cells (hESCs) are one of the most interesting cell types for tissue engineering, cell therapy, basic scientific research, and drug screening. Fast advancement in these areas requires the availability of large amounts of safe and well-characterized hESCs from hESC banks. The sensitivity of hESCs to cryopreservation, however, has severely limited the utility of these cells. It was observed that the characteristics of hESCs changed during long-term culture. Therefore, it is crucial to preserve early-passage cells by effective freezing and thawing methods. This paper reviews the current state of cryopreservation as it pertains to hESCs. We focused on the research of cryopreservarion method of hESCs.